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The carryover after extracting a sample with a high concentration of 100 ng/mL was determined for both the DBSA and the LC-HRMS/MS system and was from 0 to 18.9% (data not shown) and from 0 to 9.9%, respectively

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However, after we added a bacterial-type selenocysteine insertion sequence element to the open reading frame we successfully expressed TGR, using the strategy previously employed for production of mammalian TrxR [14,15]