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Due to the polarity of GSH, thiols, amino acids, and peptides, derivatization is often required for RP separation, extraction, or UV/fluorescence detection, using reagents like N-ethylmaleimide (NEM) [31, 50, 54], 5,5-dithiobis(2-nitrobenzoic acid) (Ellmans reagent) [33, 55], iodoacetic acid [56], orthophthalaldehyde [34, 35], monochlorobimane [49], or monobromobimane [35]

The essential role of ThDP in mitochondrial energy production (Figure 1) is universal for all tissues and therefore can hardly explain specific and primary vulnerability of the gastrointestinal system

For others, however, the structure of this type of therapy can assist with improving more significant symptoms of fatigue or brain fog

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doi: 10.1038/s41467-022-33415-5 305 Castro-MarreroJZacaresMAlmenar-PrezEAlegre-MartnJOltraE

Sophie R