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Cyst(e)ine in nutrition formulation promotes colon cancer growth and chemoresistance by activating mTORC1 and scavenging ROS

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MdxE was purified by affinity chromatography followed by size-exclusion using a Cobalt 5 mL HiTrap TALON crude column (Cytiva) and a Superdex 200 Increase 10/300 GL column (Cytiva), respectively