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An overnight culture was grown in LB-medium (1% tryptone, 1% NaCl, 0.5% yeast extract) from a freshly transformed single colony and was used to inoculate the expression batches

Jiang X, Stockwell BR, Conrad M

[DOI] [PubMed] [Google Scholar] de Keizer PL, Packer LM, Szypowska AA, Riedl-Polderman PE, van den Broek NJ, de Bruin A, Dansen TB, Marais R, Brenkman AB, Burgering BM

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Nausea, GI upset, or a temporary fishy body odor at high choline exposure