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Yu Y, Jin L, Zhuang Y, Hu Y, Cang J, Guo K

Krishnan, Anirudh

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For example, at a very conservative guestimate (taking the lowest of the range of hydrogen bond energies above 12 kJ/mole and just a thousand water molecules associated with a proteins hydrophilic domains), one arrives at a figure of around 12,000 kJ/mole for their collective energy (assuming each water molecule forms just one hydrogen bond with a neighbour or the protein: a water molecule can form up to four hydrogen bonds with its neighbours [Jorgensen and Madura, 1985])

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This process takes place primarily in the mitochondrial cristae, and when electrons leak from the ETC, they react with oxygen to form superoxide anions, a key source of mitochondrial ROS (34)