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Description

6 on/3 off (conservative)

Worms were next transferred to room temperature NGM RNAi plates (100 g/mL carbenicillin, 1 mM IPTG) that were either unseeded (fasting conditions) or seeded with freshly generated RNAi bacteria ( ad libitum control) for 24 h

PBS, phosphate buffered saline

Nasal sprays add another layer

Measurement precision matters: record the true liquid volume added, avoid rounding too early, and be aware that some syringes have dead space that can slightly affect delivered volume