5.2.1 F1 generation effects (direct offspring) The F1 generation, developing during EED exposure, exhibits the most severe reproductive impairments
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Iterative MS2 acquisitions were performed using: MS1 m/s range 1001200, MS1 scan rate 5 spectra/s, MS2 m / z range 501600, MS2 scan rate 10 spectra/s, isolate width 1.3 amu, accumulation target value 25,000 ions, active exclusion after 2 spectra for 0.2 min with a mass tolerance of 20 ppm
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The proteome results of allicin-treated infected host cells revealed an 1859% reduced abundance of the structural proteins N, M, S, and ORF3