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At day 3, the media was replaced with culture media supplemented with insulin (10 g/ml)

Assemble Your Injection Device: If you're using a syringe and vial, remove the caps and, using a new needle, draw air into the syringe equal to your dose

It depends on their health, lab results, and how they respond to treatment

[1] In practice, 1 mL (which provides the full 100 mg of B and B, etc.) is a common dose for general supplementation in deficient states, but clinicians may start with lower volumes for mild deficiencies or in smaller patients

Philadelphia, PA: Wolters Kluwer/Lippincott Williams & Wilkins

The 5 initial loading doses are usually all that is needed for long lasting protection