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(4) At the time of tumour implantation, half the animals received NMN in drinking water (2 g/L, changed twice weekly) for the remainder of the experiment

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After incubation, cells were washed with PBS twice, re-suspended, and immediately submitted to flow cytometric analysis using a BD Accuri C6 Plus flow cytometer (Becton Dickinson, Mountain View, CA, USA)

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Dihexa: the neurotrophic giant from angiotensin IV Origin and structure Dihexa (N-hexanoic-Tyr-Ile-(6) amino-hexanoic-amide, also called PNB-0408) is a modified analogue of angiotensin IV (Ang IV: Val-Tyr-Ile-His-Pro-Phe), developed in Joseph Harding's laboratory (Washington State University) from 2010-2012