After stimulation, cells were washed immediately with ice-cold PBS once before lysis and scraped down in RIPA lysis buffer complemented with 50 mM KF, 50 mM -glycerolphosphate, 2 mM EGTA (pH 8), 1 mM Na 3 VO 4 and 1 protease inhibitor cocktail (Calbiochem)
Orcid ID: orcid.org/0000-0002-9574-0436
You can also find nearby drop-offs via SafeNeedleDisposals Oregon page (lists Rogue Disposal and other programs)
Funding The present study was supported by the Noncommunicable Chronic Diseases-National Science and Technology Major Project (2024ZD0531400,2023ZD0507302), the National Key R&D Program of China (2022YFA0806101), the National Natural Science Foundation of China (81920108009, 82130023, 82570968, 82200923), the Research and Innovative Team Project for Scientific Breakthroughs at Shanxi Bethune Hospital (2024AOXIANG03), the Continuous Funding Program for High-Level Research Achievements at Shanxi Bethune Hospital (2024GSPYJ10 and 2024GSPYJ13) and the IGP Funding from QBRI, Hamad Bin Khalifa University
The impact of mass drug administration of antibiotics on the gut microbiota of target populations
baumannii (MRAB) isolates were sensitive only to amikacin