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The resulting single-stranded cDNAs were used as templates in PCR reactions using gene-specific primers to amplify TGR (forward 5-CTATTTCCGTAGACGTCTGT-3 and reverse 5-AATACAGTTTCCTTCCCGTT-3) or GAPDH (forward 5-GTTTTGGTCGTATCGGGAGA-3 and reverse 5-ATGCGTTAGAAACCACGGAC-3)

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Along with the downregulation of transcription regulators of filamentation ( TEC1 , EFG1 ), other morphogenesis-associated virulence genes ( HWP1 , ALS3 , ECE1) were also found to be affected [181]

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