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SIRT1 also prevents NF-kB activation by deacetylating the RelA/P65 subunit, thereby mitigating NF-kB downstream pro-inflammatory gene expression (Yeung et al., 2004)

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This process involves the use of reducing agents such as sodium borohydride or dithiothreitol (DTT) to break the disulfide bond in GSSG, resulting in the formation of two GSH molecules