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After appropriate treatments, cells were washed in PBS and then lysed in 100 to 150 l of buffer (100 mM NaPO 4 , 1 mM ethylenediaminetetraacetic acid (EDTA), pH 7.5) containing 0.1% Triton X-100 and frozen at -80C until analysis
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We do not perform diagnostic blood tests at CoLaz, and a private injection on top of an untested deficiency picture is not the right starting point