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HpGroEL and HpTpx are covalently bound and inhibited by Metro and Metro-P3 after reductive activation As binding of Metro and derivatives to HpGroEL and HpTpx requires the in situ activation of the nitro group under microaerophilic or anaerobic conditions, direct inhibition and binding assays with purified enzymes in vitro were challenging (Supplementary Figs

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Nosebleed, sore throat, cough and upper respiratory infections have also been reported

In this approach, a recombinant protein of interest is commonly fused to a variety of tags (including the His6-tag, maltose-binding protein, and GST) to detect, purify, and pull down the interacting partners of a protein of interest