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Tap water contains minerals, chlorine, and bacteria that will destroy the peptide and potentially cause serious infection

Application Framework: Synthesized strictly as a bulk chemical reagent for institutional laboratory experimentation, in vitro analytical research

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For Safranin O/Fast Green staining, slides were stained with hematoxylin (Mayer, Sigma) for 5 min, 0.005% Fast Green (Thermo Fisher Scientic, Pittsburgh, PA, United States) for 1 min, rinsed with 1% acetic acid, and stained with 0.1% Safranin O (Millipore, Billerica, MA, United States) for 20 min