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Tetramer staining, gating strategy and flow cytometry analysis Freshly isolated peripheral blood mononuclear cells (PBMC) (1x10 6 cells/ml) were resuspended in RPMI 1640 medium supplemented with 1% PenStrep, 10 g/ml ciprofloxacin and 10% FCS

Three technical replicates of 100 L culture were taken from each culture flask sample prior to quenching cells in cold methanol/water

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