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In line with these observations, the nitrotyrosine level in the liver of hepsin / mice was approximately 2- to threefold higher than that measured for wild-type mice, with this difference becoming noticeable as early as 2 h post-APAP administration (Fig

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Immunofluorescence staining was performed to assess macrophage polarization, with CD86 serving as a marker for pro-inflammatory M1 macrophages and CD206 for anti-inflammatory M2 macrophages