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The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes

Cytoplasmic IDH1 and mitochondrial isocitrate dehydrogenase 2 (IDH2) reversibly synthesize -ketoglutarate from isocitrate and function in the reversible mitochondrial citrate--ketoglutarate shuttle (CAS) that shuttles NADPH reducing equivalents between the cytoplasm and the mitochondrial matrix (Figure 2D)

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However, these drugs are not recommended as first-line treatment for obesity or abnormal glucose metabolism (73)