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The rats were anesthetized with intraperitoneal injection of pentobarbital sodium (50 mg/kg), and dorsal hair was shaved and depilated with hair removal cream

That same 250 mcg dose from a 2500 mcg/ml vial is only 10 units

Is sterile water with 0.9% benzyl alcohol added

If you notice any of these, discard the vial immediately

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