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To test the hypothesis that increased IGF-1 levels may mediate the effects of anti-miR-483-3p, we added IGF-1-neutralizing antibody (anti-IGF-1) to the anti-miR-483-3p-transfected dNK cell culture to neutralize the expression of IGF-1

Cells were incubated in 5 M mito-FerroGreen for 30 min at 37 C

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Maintaining an optimal maternal condition is essential for achieving favorable pregnancy rates, high-quality embryos, and ultimately, successful gestation and lactation to ensure the production of healthy calves