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Timing: Evening administration preferred

The primary antibodies (Supplemental File 2 Table 2) were diluted in block, added to the membranes, and incubated overnight at 4 C

Copper concentrations do not usually exceed 3500 g/g dry weight

The WNT protein family consists of glycoproteins that are secreted and bind to Frizzled receptor (FZD) proteins, which have seven transmembrane segments

While some small studies suggest benefits in oxidative stress or specific disease states, the routine use for general wellness or immune boosting remains largely unproven, and dosing or safety protocols vary across providers