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After incubation, cells were washed twice with PBS and solubilized in 1.0 ml detergent solution (0.1 M NaOH and 0.1% SDS) by slowly scrapping the monolayer with pipette tip and flushing the detergent solution several times

Marreiros A

[8] Similarly, patients with gout or hyperuricemia should avoid high niacin intake, as niacin (even in nicotinamide form) can raise uric acid levels and potentially trigger gout flares

Keeping certain pathways open in the detoxification process is pivotal in helping the body to remove certain waste and toxins

RPE is particularly susceptible to oxidative stress due to its role in phagocytosis of photoreceptor outer segments 2

Sodium chloride or other tonicity agents are optionally used to adjust tonicity, if necessary