An alternative interpretation for the first phase could be a rapid noncovalent interaction between PfGrx and GSSG in the vicinity of roGFP2, however, this seems less likely considering the smaller values for the structurally similar PfGrx mutants or, in particular, the rxYFP fusion constructs with ScGrx1 (Table 1)
J.PirmohamedM.et al (1998)
At 48 h post-transfection, cells were harvested in ice-cold PBS using Trypsin-EDTA (Lonza, Switzerland), centrifuged at 1000 g for 3 min, and washed with ice-cold PBS
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10.1155/2015/426069 Oxid Med Cell Longev
GCL-catalyzed conjugation of the -carbonyl group of glutamate with the amino moiety of cysteine is the rate-limiting step in glutathione synthesis