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Immunoblots revealed that HL-60 cells lack LRP1, and immunocytochemistry established that HPX trafficked with transferrin and transferrin receptor 1 (TfR1) in Rab5-positive early endosomes, supporting a clathrin-mediated endocytotic pathway used by TfR1s

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Initial detection may occur through: Blood tests showing elevated liver enzymes (ALT, AST, GGT), though normal results do not exclude fatty liver disease Ultrasound scanning revealing increased liver echogenicity (though sensitivity is limited for mild steatosis) FibroScan (transient elastography) measuring liver stiffness (to estimate fibrosis) and controlled attenuation parameter (CAP, to estimate fat content)

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