The PVDF membrane was subsequently washed (5 min 5) with tris-buffered saline with tween (TBST) buffer and then incubated with the appropriate secondary antibody (Peroxidase-conjugated goat anti-rabbit IgG (H + L), at a dilution of 1:5000) in a 5% bovine serum albumin solution for 1 h at 25 C
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Any disagreements between the first two reviewers regarding extracted data were resolved through discussion by a third reviewer
Many times, creams and serums work only on the surface, while the real issue lies deepercaused by factors like pollution, stress, poor diet, and sun exposure
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The contrasting poorer response in MASLD, where improvement rates were lower and MELD-3 more unfavourable, is also consistent with the literature
