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Sci Rep, 2016

Meanwhile, organoids were collected according to the above protocol, except that after the initial centrifugation step, the organoids were resuspended in 3 mL TrypLE Express containing 10 M Y-27632 and incubated in a 37 C water bath for 10 min, followed by trituration 10 times with a P1000 pipette followed by a P10 tip attached to the P1000 tip

Resistance Fold was calculated by dividing the IC 50 value of a substrate drug into drug-resistant SKOV3/MRP7 cells in the presence or absence of an inhibitor (sertraline, fluoxetine, citalopram, or cepharanthine) by the IC 50 value of parental SKOV3 cells without an inhibitor

RNA sequencing and data analysis RNA integrity was checked using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA)

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The body stores B12 primarily in the liver, which contains approximately 50-90% of the body's B12 reserves