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Within the first hour of nitrogen limitation, the transcription of both msmeg_5442 and msmeg_4699 was statistically significantly down-regulated by a relative factor of 2.00 (calculated by 1/expression ratio)

ND, no detection

The concentration of Human TNNI3 in the samples is then determined by comparing the OD of the samples to the standard curve

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ACKNOWLEDGMENTS The Alzheimer's Association acknowledges the contributions of Joseph Gaugler, PhD, Bryan James, PhD, Tricia Johnson, PhD, Jessica Reimer, PhD, Kezia Scales, PhD, Sarah Tom, PhD, MPH, Jennifer Weuve, MPH, ScD, and Jarmin Yeh, PhD, MPH, MSSW, in the preparation of 2025 Alzheimer's Disease Facts and Figures

The mice were traceable for quality and health status, and had not undergone any procedures before