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This study is funded by the Department of Biotechnology (DBT), Ministry of Science and Technology, Government of India (BT/PR32391/MED/30/2235/2020)

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Multiple needle punctures through the stopper are preferable to repeated stopper removal and replacement

[38] Severe MTHFR deficiency [edit] Severe MTHFR deficiency is rare (about 50 cases worldwide) and caused by mutations resulting in 020% residual enzyme activity

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The data that support the findings of this study are available from the corresponding author upon reasonable request