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hTERT RPE-1 cells were cultured in DMEM/F-12 (containing L-glutamine, HEPES buffer and sodium bicarbonate, Sigma-Aldrich) supplemented with 10% (v/v) FBS (Thermo Fisher Scientific), 100 U ml 1 penicillin (Thermo Fisher Scientific), 100 g ml 1 streptomycin (Thermo Fisher Scientific) and 0.1 mg ml 1 hygromycin B (Roth)

One liter of saline and B vitamins

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Handle with appropriate safety protocols, as it is for use by qualified professionals in controlled environments.

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