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BEAS-2B cells were treated with WS-23 (4.5, 1.5, 0.45 and 0.15 mg/mL) for 8 h, then rinsed with PBS 3 times, fixed with 4% paraformaldehyde for 10 min, then rinsed again 2 with PBS

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While there isnt much research into why, theories for this include inhaling too deeply, or the sudden change in temperatures if you go outdoors to smoke