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The relative expression levels of each gene were quantitated using the 2 CT method and normalized to the amount of endogenous glyceraldehyde-3-phosphate dehydrogenase (GAPDH)

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For example, ECM proteins crucial for cartilage structure and maintenance, such as ACAN, DCN, HSPG2, BGN, and FBLN1 were downregulated in the conditioned medium of H 2 O 2 -treated cells compared to those in control cells