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So if you know your protein of interest is happy in another buffer, you can likely use that for the binding buffer

Actively slow yourself down and aim for a 10-15 second injection time

Izzotti, A., Longobardi, M., Cartiglia, C

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Interestingly, the metabolomics results showed that the synergistic effect was related to metabolic disorders, especially the substantial influence on glycerophospholipid metabolism, which significantly increased membrane permeability

[DOI] [PubMed] [Google Scholar] 90.Konosic S., Petricevic M., Ivancan V., Konosic L., Goluza E., Krtalic B., Drmic D., Stupnisek M., Seiwerth S., Sikiric P