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[2] Exposure to nitrous oxide (including recreational use) can cause functional B12 deficiency and worsen neurological manifestations

After 48 h incubation of confluent monolayers with cARLA, the culture medium was removed and hECs were fixed with a 1:1 mixture of ice cold methanol-acetone solution for 2 min

Mohn F, Weber M, Rebhan M, Roloff TC, Richter J, Stadler MB et al

doi: 10.1038/s41467-021-26850-3 128 FungS.ShirleyM

these loci presumably act in part by regulating uric acid production and, in some cases, renal urate disposition

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