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People exposed to nitrous oxide (medical or recreational use) may develop functional B12 deficiency, as the gas inactivates B12-dependent enzymes, potentially requiring supplementation

J Epidemiol Community Health

Physical symptoms may include weakness, pale or yellowish skin, numbness or tingling in extremities, balance difficulties, mood changes including depression or irritability, and tongue inflammation

After that window, the preservative becomes less reliable, and the risk of contamination goes up

Youll want to apply a clean, wet cloth that youve cooled down for this method

It was first discovered by scientists who were researching how to treat stomach ulcers and has since been researched as far as its effects on tendons