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Beads were washed three times with 5 ml of wash buffer (50 mM NaH 2 PO 4 , 300 mM NaCl, 20 mM imidazole, 1 g ml 1 of leupeptin, 1 g ml 1 of AEBSF HCl and 1 l benzonase, pH 8.0)

The patient requires a structured loading-dose schedule followed by ongoing injections

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Br J Haematol 98(4):913-21, 1997

Joao Braga De Abreu Neto, Federal University of Rio Grande do Sul, Brazil

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