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The principle of this reaction is based on the reaction between the thiol group (-SH) of glutathione and the DTNB reagent, which results in the formation of a colored product

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In particular, up-regulation of the transcripts for the rate-limiting enzyme of the TCA cycle, 2-oxoglutarate dehydrogenase (OGDH), and for the TCA-cycle-affiliated proteins, such as subunits of pyruvate dehydrogenase (PDH) and branched chain 2-oxo acids dehydrogenase (BCODH) is observed

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