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Smith DB, Johnson KS: Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

The morphological changes were then observed using electron microscopy

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Plotting of absorbance at 283 nm vs stoichiometric equivalents of Cu(NO 3 ) 2 showed a clear hyperchromic trend with an inflection point calculated at 2.93 equivalents of Cu(NO 3 ) 2 , further supporting the formation of the trinuclear complex, Cu 3 -TC-Py