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Conclusion Determining an exact puff-to-cigarette nicotine equivalence is challenging due to individual differences in vaping and smoking behaviours

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If youre used to American blends, which often mix different tobacco types like Burley and Oriental, Canadian cigarettes might feel stronger or more straightforward

Next, we used the cleanup.genes function from the BayesPrism R package [52] to further exclude ribosomal, mitochondrial, and sex-chromosome genes, as per package instructions, as well as genes expressed in less than 50 cells