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After tons of research, I found that 1 cigarette, similar to the ones i smoked contained about 0.5 to 0.8 milligrams of nicotine, equaling roughly 20 to 24 milligrams per pack

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Cells were then blocked with 5% normal swine serum for 1 h at room temperature prior to overnight primary antibody incubation at 4 C using anti-H2AX (1:400

We then replaced it with modern foam core material

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