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Protein lysis and LEGENDplex assay Proteins from CuCl 2 -treated HepG2 and HepG2 ATP7B-KO cells were lysed by plating 500,000 cells in 800 l medium in 12-well plates

At 10 nM concentration in intestinal cells, KPV cuts NF-B-driven reporter activity significantly compared to IL-1 stimulation alone