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3 RACE Total RNA was isolated using Trizol from pPASPORT-transfected cells and 1 g of total RNA was used to perform reverse transcription using 3RACE-RT primer and M-MLV (Promega) in 20 l reaction

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This meticulous process guarantees high purity and consistency, which is absolutely vital for reliable and reproducible research outcomes

51 :102262 Corresponding authors: Qi Feng and Zhangsuo Liu, Research Institute of Nephrology, Zhengzhou University, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China

First, a weighted combination of receiver channels was conducted, followed by removal of motion-corrupted signal averages, frequency and phase drift correction using spectral registration [51], and subspectra alignment before subtraction and signal averaging were performed