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Smoking remains the largest cause of lung cancer, with 72% of cases in the UK caused by smoking

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PC1 is derived as a weighted sum of the standardized NMPs, where each parameters loading indicates its contribution to PC1s variance: 0.4535 for Nuclear Area, 0.4581 for Nuclear Perimeter, 0.4415 for Feret Diameter, 0.4563 for Raw Integrated Density, and 0.4259 for Nuclear Roundness Factor

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SAVA software was used to calculate the CBF from whole field analysis (WFA) to avoid selection bias 27