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Following chronic PNE, high affinity nicotine binding in the neonatal and fetal brain is increased, providing evidence that nicotine reaches the fetal brain and upregulates nAChR receptors [3133]

(2) SMG cells: dataset integration was carried out with the top 3000 genes (although all integrated expression was calculated for all genes, given sufficient computational resources to do this) and with k.filter = 60, UMAP was created with n.neighbors = 50, min.dist = 0.3, and SNN clustering was done using the Louvain algorithm, k.param = 30, and resolution = 0.1

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