In brief, the protocol consists of the following steps: (1) generation of flexible regions in FOXO4 FH (residues 86-104 and 186-207), the p53 TAD2 peptide, and spin labels, randomization of the flexible regions
Stop at least 2 months before a planned pregnancy because of the long half-life
The selected particles were then subjected to reference-based motion correction in cryoSPARC (v4.5.0), followed by several rounds of heterogeneous refinement using volumes of different states of SLC33A1, resulting in a final stack of 215,071 particles
(5) Adverse effects and management: Cholinergic muscarinic effects such as diarrhea, abdominal cramping, and increased salivation are common and may be managed with agents such as propantheline or loperamide
What is the Wolverine stack
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