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In general, under normal conditions, endogenous ROS is sustained at low concentrations by intracellular redox equilibrium

Reaction: GSH + ROS GSSG (oxidized glutathione) + HO GSSG is then recycled back to GSH by glutathione reductase, using NADPH

doi: 10.1038/nri3863 97 MansourDHofmannAGemzell-DanielssonK

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Next, Ingenuity Pathway Analysis was used to identify potential upstream regulators that predict the observed mRNA changes

Tumor volume and body weight were measured every other day until day 14