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Unlike NSAIDs that block a single enzyme, therapeutic peptides target multiple interconnected mechanisms

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To investigate whether inhibition of Gpx induces T-cell hyporesponsiveness, we pretreated 0.4 mM MS (Gpx inhibitor, Sigma-Aldrich) for 48 h, then treated Con A (5 g/ml) for 8 h in Jurkat T cells

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Besides a unique antibody that specifically recognizes palmitoylated PSD-95 37 , experimental approaches to study zDHHC-substrate pair relations are based exclusively on disruptive biochemical and proteomic techniques 38