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The suggested dithiol mechanism was based on experiments at a single substrate concentration that required more than 8 h for redox equilibration in the presence of 0.5 M glutaredoxin with or without 1 M GSH
All MD simulations were performed with the program package GROMACS (version 2020.4) 32 using the GROMOS 54a7 force field 33
Nbetler: Ciddi glutatyon eksiklii, beyindeki oksidatif stresi artrarak nbetlere neden olabilir
The observed inconsistencies between available in planta, in vitro and in vivo (cell factory) data, however, led us to hypothesize that arGSTs might not (only) have the literature-proposed transport function but could play a central role in the biosynthetic step from leucoanthocyanidins ( 2 ) to anthocyanidins ( 3 )