It is primarily synthesized in the liver and is crucial for detoxification, immune function, and cellular repair
toll-like receptor-4 (TLR4) (dilution 1:1,000) (Santa Cruz Biotechnology, Inc., sc-293072), nuclear factor erythroid 2-related factor 2 (Nrf2) (dilution 1:500), nuclear factor-kappa B (NF-B p65) (dilution 1:500), cleaved caspase-3 (c-caspase-3) (dilution 1:500) (Chongqing Biospes Co., Ltd., China, YPA1865, BBP1066, and YPA2210, respectively) and -actin (dilution 1:3,000) (Elabscience Biotechnology, Inc., E-AB-20031)
Unextracted plasma was directly subjected to EIA, demonstrating displacement curves parallel to the standards
doi: 10.3390/biomedicines12092121 109 PawaseP
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