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Combined with clinical biomarkers and health history, this supports more personalized supplementation decisions
Cell pellets were resuspended in reducing lysis buffer (20 mM potassium phosphate, 200 mM NaCl, pH 7.4, 10 mM DTT, 0.01 mg ml 1 DNase I) to an OD 600 of 140 and lysed by two rounds of sonication for 2 min at 50% amplitude with 2 s intervals using a Sonopuls (Bandelin) sonicator
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Lipids 7:518524 Coelho DMN, Costa Jnior DC, da Silva DMA, Alves ACB, Chaves RC, Rebouas MO, Valentim JT, de Oliveira AA, Sales ISL, Nicolau LAD, de Sousa FCF (2024) Long-term administration of omeprazole in mice: a study of behavior, inflammatory, and oxidative stress alterations with focus on central nervous system
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