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Molecular mediators governing iron-copper interactions

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The cells were collected 48 h post-transfection, washed twice with PBS and lysed in 1 ml of HEPES buffer (20 mM HEPES-KOH (pH 7.9), 300 mM KCl, 0.2 % NP-40, 15 % glycerol, 1 mM DTT and protease inhibitors, Sigma-Aldrich, P8340) for 20 min

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Importantly, these pregnancy complications encompass a range of pathologies with different etiologies

These data confirm, on the genome level, Human-SFB-ML as a new SFB species and, to our knowledge, identify humans as the first host species harboring multiple SFB species